Competitive Exclusion of Epiphytic Bacteria by Ice − Pseudomonas syringae Mutants
نویسندگان
چکیده
منابع مشابه
Production of Ice Nucleation Deficient (Ice-) Mutants of the Epiphytic Strains of Erwinia herbicola
To mutate the Ice Nucleation Active (INA) gene in Erwinia herbicola strains, Tn-5 transposon carried by Psup2021 plasmid was used. This plasmid was transferred to the bacterial cells by electroporation. Electrotransformation was carried out for 2.5 ms at 1800 v and 1 mm distance between the electrodes. Polymerase chain reaction was used for determination of presence or loss of INA gene, using a...
متن کاملEpiphytic Ice Nucleation-Active Bacteria
Wheat straw smoke aerosols and liquid smoke condensates reduced significantly both the viability and the ice-nucleating activity of Pseudomonas syringae pv. syringae and Erwinia herbicola in vitro and on leaf surfaces in vivo. Highly significant reductions in numbers of bacterial ice nuclei on the surface of both corn and almond were observed after exposure to smoke aerosols. At -5°C, frost inj...
متن کاملCharacteristics of Insertional Mutants of Pseudomonas syringae with Reduced Epiphytic Fitness.
Random Tn5 mutagenesis was used to identify genes ir. Pseudomonas syringae which contribute to epiphytic fitness. Mutants were selected on the basis of deficiencies in epiphytic growth or survival on plants rather than deficiencies in predetermined phenotypes exhibited in culture. A sample freezing procedure was used to measure the population sizes of 5,300 mutants of P. syringae exposed to alt...
متن کاملبررسی اثر چند ژن بیماری زا در زندگی اپیفیتی (Pseudomonas syringae)
Pseudomonas syringae is a phytopathogenic bacterium with a wide host range. The biology of this bacterium consists of two phases. The first phase is the indication of disease on the host plant which generally appears in the form of necrosis on the aerial parts of plant (pathogenicity phase). The second phase is a rapid multiplication of bacteria on the aerial surface of the plant without inflic...
متن کاملبررسی اثر چند ژن بیماری زا در زندگی اپیفیتی (Pseudomonas syringae)
Pseudomonas syringae is a phytopathogenic bacterium with a wide host range. The biology of this bacterium consists of two phases. The first phase is the indication of disease on the host plant which generally appears in the form of necrosis on the aerial parts of plant (pathogenicity phase). The second phase is a rapid multiplication of bacteria on the aerial surface of the plant without inflic...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1987
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.53.10.2520-2527.1987